Effects of four components of the polyene antibiotic, filipin, on phospholipid spherules (liposomes) and erythrocytes.

Stoichiometry of interaction of the polyene antibiotic, filipin, with free and liposomal-bound cholesterol.

Investigation of Channel-Forming Activity of Polyene Macrolide Antibiotics in Planar Lipid Bilayers in the Presence of Dipole Modifiers

The role of membrane components, sterols, phospholipids and sphingolipids in the formation and functioning of ion-permeable nanopores formed by antifungal macrolide antibiotics, amphotericin B, nystatin and filipin in planar lipid bilayers was studied. Dipole modifiers, flavonoids and styryl dyes, were used as a tool to study the molecular mechanisms of polyene channel-forming activity. The int...

Mode of hemolytic action of the antifungal polyene antibiotic filipin.

Filipin at low concentrations rapidly hemolyzes bovine erythrocytes. Of the compounds tested, which were known a constituents of cell membranes, only cholesterol and phosphatidyl-choline prevented hemolysis. A specific complex was formed only with cholesterol. Filipin treatment caused a release of cholesterol from erythrocytes. Since all cell sterol is located in the cell membrane, the hemolyti...

Classification of polyene antibiotics according to chemical structure and biological effects.

Fourteen polyene antibiotics and six of their semisynthetic derivatives were compared for their effects on potassium (K(+)) leakage and lethality or hemolysis of either Saccharomyces cerevisiae or mouse erythrocytes. These polyene antibiotics fell into two groups. Group I antibiotics caused K(+) leakage and cell death or hemolysis at the same concentrations of added polyene. In this group fungi...

The Effect of Polyene Antibiotic Filipin on Pig Red Blood Cells

Polyene antibiotic filipin (Fil) is a potent hemolytic agent. Our previous kinetics analysis showed that the mechanism of Fil action on human erythrocytes can be classified as the damage type. Results of other experiments performed by us demonstrated that the kinetics of hemolysis induced in RBCs by two other polyene antibiotics, namely amphotericin B (AmB) and nystatin (Nys), is more complex, ...